doc. Mgr. Tomáš Doležal, Ph.D.

head of the Laboratory of Molecular Integrative Physiology in Drosophila

Faculty of Science, University of South Bohemia
Branišovská 31, 37005 České Budějovice
building B, ground floor, door number 193
 
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel. +420 387772229

ORCID ID: 0000-0001-5217-4465

• Doležal T. (2023) How to eliminate pathogen without killing oneself? Immunometabolism of encapsulation and melanization in Drosophila. Frontiers in Immunology. Dec 6:14:1330312. doi: 10.3389/fimmu.2023.1330312. eCollection 2023.
• Krejčová G., Morgantini C., Zemanová H., Lauschke V.M., Kovářová J., Kubásek J., Nedbalová P., Kamps-Hughes N., Moos M., Aouadi M., Doležal T. & Bajgar A. (2023) Macrophage-derived insulin antagonist ImpL2 induces lipoprotein mobilization upon bacterial infection. The EMBO Journal. Dec 1;42(23):e114086. doi: 10.15252/embj.2023114086. Epub 2023 Oct 9.
• Bajgar A,  Krejčová G, Doležal T (2021) Polarization of Macrophages in Insects: Opening Gates for Immuno-Metabolic Research. Front Cell Dev Biol, 15 February 2021 | https://doi.org/10.3389/fcell.2021.629238 (IF=5.2)
• Doležal T, Nedbalová P, Krejčová G, Kazek M, Lehr K, Chodáková L, Strych L, Dolejšková T, Bajgar A (2020) Privileged immune cell upon activation – how it changes its own metabolism and metabolism of the whole organism.  figshare POSTER DOI: 10.6084/m9.figshare.12144831.v1
• Krejcova G, Danielova A, Nedbalova P, Kazek M, Strych L, Chawla G, Tennessen JM, Lieskovská J, Jindra M, Dolezal T, Bajgar A (2019) Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense. eLife 14;8. pii: e50414. doi: 10.7554/eLife.50414. (IF=7.5)
• Bajgar A, Salon I, Krejcová G, Dolezal T, Jindra M, Stepanek F (2019) Yeast glucan particles enable intracellular protein delivery in Drosophila without compromising the immune system. Biomater Sci 30. doi: 10.1039/c9bm00539k. (IF=5.2)
• Dolezal T, Krejcova G, Bajgar A, Nedbalova P, Strasser P (2019) Molecular regulations of metabolism during immune response in insects. Insect Biochem Mol Biol 109:31-42. doi: 10.1016/j.ibmb.2019.04.005. (IF=3.9)
• Bajgar A, Dolezal T (2018) Extracellular adenosine modulates host-pathogen interactions through regulation of systemic metabolism during immune response in Drosophila. PLoS Pathog 14(4): e1007022 (IF=6.6)
• Dolezal T (2015) - Adenosine: a selfish-immunity signal? Oncotarget - Immunology and Microbiology Section 6 (32), 32307-32308 (IF=6.3)
• Bajgar A, Kucerova K, Jonatova L, Tomcala A, Schneedorferova I, Okrouhlik J, Dolezal T (2015) Extracellular Adenosine Mediates a Systemic Metabolic Switch during Immune Response. PLoS Biol 13(4): e1002135 (IF=11.8)
• Novakova M and Dolezal T (2011). Expression of Drosophila adenosine deaminase in immune cells during inflammatory response. PLoS ONE 6(3): e17741 (IF=3.2)
• Fenckova M, Hobizalova R, Fric Z, Dolezal T (2011). Functional characterization of ecto-5’-nucleotidases and apyrases in Drosophila melanogaster. Insect Biochem Mol Biol 41(12): 956-967 (IF=3.4)
• Zuberova M, Fenckova M, Simek P, Janeckova L, Dolezal T (2010). Increased extracellular adenosine in adenosine deaminase deficient flies activates a release of energy stores leading to wasting and death. Dis Model Mech 3(11-12): 773-84 (IF=4.9)
• Dolezal T, Kucerova K, Neuhold J, Bryant PJ (2010). Casein kinase I epsilon somatic mutations found in breast cancer cause overgrowth in Drosophila. Int J Dev Biol 54: 1419 – 1424 (IF=2.8)
• Foldynova-Trantirkova S, Sekyrova P, Tmejova K, Brumovska E, Bernatik O, Blankenfeldt W, Krejci P, Kozubik A, Dolezal T, Trantirek L, Bryja V. (2010). Breast cancer specific mutations in CK1epsilon inhibit Wnt/beta-catenin and activate Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration. Breast Cancer Res 12(3): R30 (IF=5.5)
• Dolezal T, Dolezelova E, Zurovec M, Bryant PJ (2005). A Role for Adenosine Deaminase in Drosophila Larval Development. PLoS Biol 3(7): e201 (IF=11.8)
• Dolezelova E, Zurovec M, Dolezal T, Simek P, Bryant, PJ (2005). The emerging role of adenosine deaminases in insects. Insect Biochem Mol Biol 35(5): 381-9 (IF=2.8)
• Dolezal T, Gazi M, Zurovec M, Bryant PJ (2003). Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila. Genetics 165: 653-666 (IF=4.3)
• Zurovec M, Dolezal T, Gazi M, Pavlova E, Bryant PJ (2002). Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine. PNAS 99(7): 4403-4408 (IF=10.9)

Teaching of courses:
Fundamentals of Cell Biology (KMB/023) – guarantor and lecturer

Cellular and Molecular Biology and Genetics I (KMB/758) - guarantor and lecturer

Advanced methods in Molecular Biology (KMB/603) - guarantor and lecturer

2006-2016 guarantor and lecturer (100%) of “Foundation of modern biology” (undergraduate course)
2007-2010 guarantor and lecturer (100%) of “Molecular biology and Genetics” (undergraduate course in English for international program Biological Chemistry)
2008-2009 guarantor and lecturer (20%) of “Cell regulation and signaling” (graduate course in English)
2010-2012 guarantor and lecturer (100%) of “Basic methods in molecular biology” (undergraduate course)
since 2014 lecturer (50%) of “Developmental biology” (undergraduate course)
since 2017 guarantor and lecturer (100%) of "Introduction to Cell Biology" - mandatory course for Czech undergraduate Biology programme and undergraduate course in English for international program Biological Chemistry
since 2017 guarantor of "Methods in Molecular Biology" (undergraduate course in English for international program Biological Chemistry)

Supervision of students and postdocs

2 Postdocs / 4 Ph.D. students / 4 Master Students / 19 Bachelor Students

Ph.D. theses:
Milena Novakova, 2011 - "Regulation of extracellular adenosine level by ADGF-A in Drosophila melanogaster"
Michaela Fenckova, 2012 - "Role of extracellular adenosine in Drosophila"
Monika Zuberova, 2012 - "The role of adenosine signaling pathway in regulation of metabolic reserves"

Master degree theses:
Tomas Gregor, 2015 - "Tissue specific mutagenesis with TAL nucleases using Drosophila melanogaster as a model" (in Czech)
Lucie Jonatova, 2013 - "The Role of Dco in Drosophila Haematopoiesis”
Jana Matulova (Neuhold), 2009 - "Study of the Role of Casein Kinase I epsilon in BreastCancer Using Drosophila as a Model"

Supervised Bachelor students:
David Opavsky, Jana Kadlecova, Tomas Krucek, Radka Hobizalova, Eugen Kubala, David Hartmann, Sarka Pospisilova, Tamara Aigner, Bianca Spitzbart, Marek Renner, Kristina Preur, Tomas Gregor, Jiri Stros, Paul Strasser, Klara Kopicova, Thomas Stehrer, Barbara Jetzinger, Lukas Strych, Katharina Lehr

Principle investigator: Václav Vondrovský (Institute of Archaeology CAS)
Co-investigator from the department: Michaela Ptáková
Funding provider: Czech Science Foundation
Grant No.: 21-16614S
Duration: 2021- 2023

Peripheral zones of Neolithic occupation in Central Europe have remained out of the scope of modern archaeological research. This project is therefore focused on a specific region of South Bohemia which was colonised by a secondary wave of Linear Pottery culture and it was occupied only sporadically in the later Neolithic periods. We will study the dynamics of occupation in the region, interregional contacts, possible contacts with hunter-gatherer groups, subsistence strategies of local farmers and their interactions with the regional environment. In comparison with conventionally studied core regions of the Elbe, Danube, and Morava rivers, we strive for understanding of the Neolithic cultural habitus in more detail. This could reach its limits in peripheral zones and be subject to adaptations and considerable diversity of practice. To follow this objective, we need to enlarge current data using standard archaeological methods and multiproxy environmental approaches, such as analyses of plant macroremains, phytoliths, anthracological assemblages, and the pollen record.

Principle investigator: Jaromír Beneš
Funding provider: Interreg, European Commission
Grant No.: Cil - Ziel Interreg 343
Duration: 2021- 2023

The purpose and goal of the project are to strengthen basic research in the field of botany, ethnobotany and archaeobotany. The project aims to develop an innovative methodological procedure and scientific tool that will enable the study of current and past vegetation of the Czech-Bavarian area by means of artificial intelligence. The basis will be the development of comparative methods of plant parts that carry specific information. The project will create a digital plant macro- and microhabitats database, emphasising FAIR data principles. In the next phase, a module capable of semi-automatically identifying plant seeds will be developed for about 50 plant taxa and a pilot series of plant phytoliths and starches. The resulting methodology will serve as a tool for reconstructing past natural environments and studying human-nature interactions in the Czech-Bavarian area from prehistory to the present. The partner of the project is the University of Regensburg.

Principle investigator: David Janík (The Silva Tarouca Research Institute for Landscape and Ornamental Gardening)
Co-investigator from the department: Pavel Fibich
Funding provider: the Grant Agency of the Czech Republic
Grant No.: 23-06745S
Duration: 2023- 2025

Explaining mechanisms of coexistence in species-rich and structurally diverse forests is still a
great challenge in ecology. Niche-based processes, reflecting differences in functional traits of
neighbouring trees, provide a viable explanation of forest dynamics. This project aims to
investigate how functional traits contribute to community dynamics in temperate and tropical
forests by mediating tree interactions with neighbours, enemies, and mutualists across different
environmental contexts and spatio-temporal scales. Using several observational and
experimental approaches associated with large tropical and temperate forest plots, we plan to
explore the effects of 1) similarity of functional traits on the coexistence with neighbouring trees,
2) adult trees, natural enemies and mycorrhizal fungi on seedling performance and traits, and 3)
species- and individual-level functional traits during the regeneration phase. This will
substantially advance our understanding of the effects of tree’s functional traits for the
coexistence of tropical and temperate tree species