RNDr. Alena Krejčí, Ph.D.

head of the Laboratory of Bee Biology

Faculty of Science, University of South Bohemia
Branišovská 31, 37005 České Budějovice
building B, ground floor, door number 192
 
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel. +420 387772270

ORCID ID: 0000-0001-7589-4035 

• Active member of the local branch of the Czech beekeeping society and a hobby beekeeper (15 hives)
• Co-manager of Experimental and educational apiary at the Biology Centre, Ceské Budějovice (30 hives)
• Popular bee science talks to students and public
• TV and radio programmes about bees, newspaper interviews
• Current grant from the Technology Agency of the Czech Republic for antiviral effects of natural ingredience in bee keeping
• Collaboration with the Department of robotics and embeded systems at the University of south Bohemia - designing a beehive themperature and CO₂ sensors, image analysis using neural networks and machine learning

• Mentor of 2 succesfully defended PhD students (plus 1 writing), 4 master students and 14 bachelor students.

• Molecular biology course (undegrad)
• Developmental bIology course (undergrad)

• Bruce Krejčí A, Krištůfek V: Mravenčí trápení (Tha ant's sorrow). Moderní včelař (Modern Beekeeper) 03, 34-35, 2022.
• Bruce Krejčí A, Svobodová K, Krištůfek V: Testování kvality vosku stanovením jeho teploty tuhnutí (Testing the wax quality by assesing its setting temperature). Moderní včelař (Modern Beekeeper) 02, 16-18, 2022.
• Krištůfek V, Bruce Krejčí A, Svobodová K, Chalupský J: Snižují houby virózy a úmrtnost včel? (Do mushrooms lower virus loads and mortality of honey bee colonies?)  Moderní včelař (Modern Beekeeper) 06, 28-30, 2021.
• Urajová P., Krištůfek V., Bruce Krejčí A: Kyselina para-kumarová a výživa včel (Para-coumaric acid and bee nutrition). Moderní včelař (Modern Beekeeper) 07, 28-30, 2020.
• Perez-Gomez R, Perez-Gomez R, Magnin V, Mihajlovic Z, Slaninova V and Krejci A: Downregulation of respiratory complex I mediates major signalling changes triggered by TOR activation. Scientific Reports 10, 4401, 2020.
• Mihajlovic Z, Tanasic D, Bajgar A, Perez-Gomez R, Steffal P, Krejci A: Lime is a new protein linking immunity and metabolism in Drosophila. Developmental Biology 452(2):83-94, 2019.
• Krejci A, Tennessen JM.: Metabolism in time and space - exploring the frontier of developmental biology. Development 144(18):3193-3198, 2017.
• Horvath M, Mihajlovic Z, Slaninova V, Perez-Gomez R, Moshkin Y, Krejci A: The silent information regulator 1 (Sirt1) is a positive regulator of the Notch pathway in Drosophila. Biochemical journal 473(22):4129-4143, 2016.
• Slaninova V, Krafcikova M, Perez-Gomez R, Steffal P, Trantirek L, Bray SJ and Krejci A: Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. Open Biology, 2016 Feb;6(2)., 2016. doi: 10.1098/rsob.150155.
• Housden BE, Fu AQ, Krejci A, Bernard F, Fischer B, Tavare S, Russell S, Bray SJ: Transcriptional Dynamics Elicited by a Short Pulse of Notch Activation Involves Feed-Forward Regulation by E(spl)/Hes Genes. Plos Genetics, 9(1):e1003162, 2013.
• Djiane A, Krejci A, Bernard F, Fexova S, Millen K, Bray SJ.: Dissecting the mechanisms of Notch induced hyperplasia.  EMBO Journal 32, 60 - 71, 2013. doi:
• Endo K, Karim MR, Taniguchi H, Krejci A, Kinameri E, Siebert M, Ito K., BraySJ, Moore AW: Chromatin modification of Notch targets in olfactory receptor neuron diversification. Nature Neuroscience, 15(2):224-33, 2012. doi: 10.1038/nn.2998.
• Terriente-Felix A, Li J, Collins S, Mulligan A, Reekie I, Bernard F, Krejci A,  Bray SJ: Notch co-operates with Lozenge/Runx to lock hemocytes into a differentiation programme. Development, 140(4):926-37, 2013. doi: 10.1242/dev.086785
• Bernard F, Krejci A, Housden B, Adryan B, Bray SJ: Specificity of Notch pathway activation: twist controls the transcriptional output in adult muscle progenitors. Development, 137(16):2633-42, 2010. doi: 10.1242/dev.053181
• Krejci A*, Bernard F*, Housden BE*, Collins S, Bray SJ.: Direct response to Notch activation: signaling crosstalk and incoherent logic. Science Signal.,2(55), 2009. doi:10.1126/scisignal.2000140
• Narasimha M, Uv A, Krejci A, Brown NH, Bray SJ.: Grainy head promotes expression of septate junction proteins and influences epithelial morphogenesis. J Cell Sci. 2008. doi: 10.1242/jcs.019422
• Goodfellow H*, Krejcí A*, Moshkin Y*, Verrijzer CP, Karch F, Bray SJ.: Gene-specific targeting of the histone chaperone asf1 to mediate silencing. Dev Cell.13(4):593-600, 2007.PMID: 17925233
• Krejci A and Bray S: Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers.  Genes Dev. 21(11):1322-7, 2007. PMID: 17545467
• Bruce AW, Krejci A, Ooi L, Deuchars J, Wood IC, Dolezal V, Buckley NJ. :The transcriptional repressor REST is a critical regulator of the neurosecretory phenotype. J Neurochem. 98(6):1828-40, 2006. PMID: 16945103
• Nagel AC, Krejci A, Tenin G, Bravo-Patino A, Bray S, Maier D, Preiss A. : Hairless-mediated repression of notch target genes requires the combined activity of Groucho and CtBP corepressors. Mol Cell  Biol. 25(23):10433-41, 2005. PMID: 16287856
• Jakubik J, Krejci A, Dolezal V: Asparagine, valine, and threonine in the third extracellular loop of muscarinic receptor have essential roles in the positive cooperativity of strychnine-like allosteric modulators. J Pharmacol Exp Ther. 313(2):688-96, 2005.
• Krejci A, Bruce AW, Tucek S, Dolezal V, Buckley NJ: Multiple promoters drive tissue specific expression of the human M₂ muscarinic acetylcholine receptor gene. J Neurochem. 91(1):88-98, 2004.
• Tucek S, Krejci A, Lysikova M, Jakubik J, Dolezal V: Roles of external loops of muscarinic receptors in interactions between N-methylscopolamine and allosteric modulators. In Cholinergic mechanisms: Function and disfunction. Ed. I. Silman, 71-76, 2004.
• Krejci A, Michal P, Jakubik J, Ricny J, Dolezal V: Regulation of signal transduction at M₂ muscarinic receptor. Physiol. Res., 53, suppl.1:S131-40, 2004.
• Krejci A, Tucek S: Quantitation of mRNAs for M₁ to M₅ subtypes of muscarinic receptors in rat heart and brain cortex. Mol Pharmacol.  61(6):1267-72, 2002.
• Krejci A, Tucek S: Changes of cooperativity between N-methylscopolamine and allosteric modulator alcuronium and gallamine induced by mutations of external loops of muscarinic M₃ receptors. Mol. Pharmacol. 60 : 761-767, 2001.

prof. Alexander William Bruce, Ph.D.

head of the Laboratory of Early Mammalian Developmental Biology (LEMDB)

Faculty of Science, University of South Bohemia
Branišovská 31, 37005 České Budějovice
building B, ground floor, door number 182
 
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel. +420 387772291

ORCID ID: 0000-0003-4297-4412

• Mani RR, Raj S, Bruce AW, Anbalagan M, Srinivasan H, Chinnappan S, Rajagopal M, Khanna K, Chandramoorthy CK. COVID-19 influenced gut dysbiosis, post-acute sequelae, immune regulation, and therapeutic regimens. Front. Cell. Infect. Microbiol. In press (2024). IF₂₀₂₃ = 4.6
• Fluks M, Collier R, Walewska A, Bruce AW, Ajduk A. How great thou ART: Biomechanical properties of oocytes and embryos as indicators of quality in assisted reproductive technologies. Front. Cell Dev. Biol. 12:1342905 (2024). IF₂₀₂₃ = 4.6
• Iyyappan R, Aleshkina D, Ming H, Dvoran M, Kakavand K, Jansova D, Del Llano E, Gahurova L, Bruce AW, Masek T, Pospisek M, Horvat F, Kubelka M, Jiang Z, Susor A. The translational oscillation in oocyte and early embryo development. Nucleic Acids Res. gkad996 (2023). IF₂₀₂₃ = 16.6
• Gahurova L, Tomankova J, Cerna P, Bora P, Kubickova M, Virnicchi G , Kovacovicova K, Potesil D, Hruska P, Zdrahal Z, Anger M, Susor A and Bruce AW. Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m7G-cap dependent translation at the 8- to 16-cell transition. Open Biology 8:230081 (2023). IF₂₀₂₃ = 4.5
• Bora P, Gahurova L, Hauserova A, Stiborova M, Collier R, Potěšil D, Zdráhal Z and Bruce AW. DDX21 is a p38-MAPK sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification. Open Biology 7:210092 (2021). IF₂₀₂₁ = 7.000.
• Bora P, Gahurova L, Mašek T, Hauserova A, Potěšil D, Jansova D, Susor A, Zdráhal Z, Ajduk A, Pospíšek M and Bruce AW. p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice. Commun. Biol. 4:788 (2021). IF₂₀₂₁ = 6.548.
• Virnicchi G, Bora P, Gahurova L, Šušor A and Bruce AW. Wwc2 is a novel cell division regulator during preimplantation mouse embryo lineage formation and oogenesis. Front. Cell. Dev. Biol. 8:857 (2020). IF₂₀₂₀ = 6.684.
• Del Llano E, Masek T, Gahurova L, Pospisek M, Koncicka M, Jindrova A, Jansova D, Iappan R, Roucova K, Bruce AW, Kubelka M and Susor A. Age-related differences in the translational landscape of mammalian oocytes. Aging Cell e13231 (2020). IF₂₀₂₀ = 9.304.
• Masek T, Del Llano E, Gahurova L, Kubelka M, Susor A, Roucova K, Lin C-J, Bruce AW and Pospisek M. Identifying the translatome of mouse NEBD-stage oocytes via SSP-profiling; a novel polysome fractionation method. Int. J. Mol. Sci. 21(4), 1254 (2020). IF₂₀₂₀ = 5.923.
• Bora P, Thamodaran V, Šušor A and Bruce AW. p38-mitogen activated kinases mediate a developmental regulatory response to amino acid depletion and associated oxidative stress in mouse blastocyst embryos. Front. Cell. Dev. Biol.7:276 (2019). IF₂₀₁₉= 5.186.
• Koncicka M, Tetkova A, Jansova D, Del Llano E, Gahurova L, Kracmarova J, Prokesova S, Masek T, Pospisek M, Bruce AW, Kubelka M and Susor A. Increased expression of maturation Promoting Factor components speeds up meiosis in oocytes from aged females. Int. J. Mol. Sci. 19(9), 2841 (2018). IF₂₀₁₈ = 4.183.
• Mihajlović AI and Bruce AW. The first cell-fate decision of mouse preimplantation embryo development: integrating cell position and polarity. Open Biology. 7(11) /rsob.170210 (2017). IF₂₀₁₇ = 3.286.
• Koštál V, Štětina T, Poupardin R, Korbelová J, Bruce AW. Conceptual framework of the eco-physiological phases of insect diapause development justified by transcriptomic profiling. Proc. Natl. Acad. Sci. USA. 114(32): 8532-8537 (2017). IF₂₀₁₇ = 9.504.
• Thamodaran V and Bruce AW. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development. Open Biology. 6(9) /rsob.160190 (2016). IF₂₀₁₆ = 3.481.
• Mihajlović AI and Bruce AW. Rho-associated protein kinase regulates subcellular localisation of Angiomotin and Hippo-signalling during preimplantation mouse embryo development. Reprod. Biomed. Online. 33(3), 381-390 (2016). IF₂₀₁₆ = 3.249.
• Mihajlović AI, Thamodaran V and Bruce AW. The first two cell-fate decisions of preimplantation mouse embryo development are not functionally independent. Sci. Rep. 13(5): 15034 (2015). IF₂₀₁₅ = 5.228.
• Bruce AW. Generating different genetic expression patterns in the early embryo; insights from the mouse model. Reprod. Biomed. Online. 27(6), 586-592 (2013). IF₂₀₁₃ = 2.98.
• Bruce AW. Response: Role of mouse maternal Cdx2: what’s the debate all about? Reprod. Biomed. Online. 22(6), 519-5120 (2011)b. IF₂₀₁₁ = 2.042.
• Bruce AW. What is the role of maternally provided Cdx2 mRNA in early mouse embryogenesis? Reprod. Biomed. Online. 22(6), 512-515 (2011)a. IF₂₀₁₁ = 2.042.
• Dhami P^§, Saffrey P^§, Bruce AW^§, Dillon SC^§, Chiang K, Bonhoure N, Koch CM, Bye J, James K, Foad NS, Ellis PD, Watkins NA, Ouwehand WH, Langford CF, Andrews RM, Dunham I and Vetrie D. Complex exon-intron marking by histone modifications is not determined solely by nucleosome distribution. PLoS One. 5(8), e12339 (2010). ^§equal contribution. IF₂₀₁₀ = 4.411.
• Bruce AW and Zernicka-Goetz M. Developmental control of the early mammalian embryo: competition among heterogeneous cells that biases cell fate. Curr. Opin. Genet. & Dev. 20, 485-491 (2010). IF₂₀₁₀ = 9.381.
• Jedrusik A, Bruce AW^§, Tan MH^§, Leong DE, Skamagki M, Yao M and Zernicka-Goetz M. Maternally and zygotically provided Cdx2 have novel and critical roles for early development of the mouse embryo. Dev. Biol. 344, 66-78 (2010). §equal contribution. IF₂₀₁₀ = 4.094.
• Dhami P, Bruce AW, Jim JH, Dillon SC, Hall A, Cooper JL, Bonhoure N, Chiang K, Ellis PD, Langford CF, Andrews RM and Vetrie D. Genomic approaches uncover increasing complexities in the regulatory landscape at the human SCL (TAL1) locus. PLoS One. 5(2), e9059 (2010). IF₂₀₁₀ = 4.411.
• Wu Q^§, Bruce AW^§, Jedrusik A, Ellis PD, Andrews RM, Langford CF, Glover DM and Zernicka-Goetz M. CARM1 is required in ES cells to maintain pluripotency and resist differentiation. Stem Cells. 27, 2637-2645 (2009) ^§equal contribution. IF₂₀₀₉ = 7.747.
• Zernicka-Goetz M, Morris SA and Bruce AW. Making a firm decision: Multifaceted regulation of cell fate in preimplantation development. Nat. Rev. Genet. 10, 467-477 (2009). IF₂₀₀₉ = 27.822.
• Bruce AW, Lopez-Contreras AJ, Flicek P, Down T, Dhami P, Dillon SC, Koch CM, Langford, CF, Dunham I, Andrews RM and Vetrie D. Functional diversity for REST (NRSF) is defined by in vivo binding affinity hierarchies at the DNA sequence level. Genome Res. 19, 994-1005 (2009). IF₂₀₀₉ = 11.342.
• The ENCODE Project Consortium (inc. Bruce AW). Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. Nature. 447, 799-816 (2007). IF₂₀₀₇ = 28.751.
• Koch CM, Andrews RM, Flicek P, Dillon SC, Karaoz U, Clelland GK, Wilcox S, Beare DM, Fowler JC, Couttet P, James KD, Lefebvre GC, Bruce AW, Dovey OM, Ellis PD, Dhami P, Langford CF, Weng Z, Birney E, Carter NP, Vetrie D and Dunham I. The landscape of histone modifications across 1% of the human genome in five human cell lines. Genome Res. 17, 691-707 (2007). IF₂₀₀₇ = 11.224.
• Johnson RJ, Gamblin RJ, Ooi L, Bruce AW, Donaldson IJ, Westhead DR, Wood IC, Jackson RM, Buckley NJ. Identification of the REST regulon reveals extensive transposable element-mediated binding site duplication. Nucleic Acids Res. 34, 3862-3877 (2006). IF₂₀₀₆ = 6.317.
• Bruce AW, Krejčí A, Ooi L, Deuchars J, Wood IC, Doležal V and Buckley NJ. The transcriptional repressor REST is a critical regulator of the neurosecretory phenotype. J. Neurochem. 98, 1828-1840 (2006). IF₂₀₀₆ = 4.260.
• Follows GA, Dhami P, Göttgens B, Bruce AW, Campbell PJ, Dillon SC, Smith AM, Koch C, Donaldson IJ, Scott MA, Dunham I, Janes ME, Vetrie D, Green AR. Identifying gene regulatory elements by genomic microarray mapping of DNaseI hypersensitive sites. Genome Res. 16, 1310-1319 (2006). IF₂₀₀₆ = 10.256.
• Bruce AW^§, Donaldson IJ^§, Wood IC, Yerbury SA, Chapman M, Sadowski MI, Göttgens B and Buckley NJ. Genome-wide analysis of Repressor Element-1 Silencing Transcription factor (REST) binding sites reveals novel target genes. Proc. Natl. Acad. Sci. USA. 101, 10458-10463 (2004). ^§equal contribution. IF₂₀₀₄ = 10.452.
• Krejčí A, Bruce AW, Tuček S, Doležal V, Buckley NJ. Multiple promoters drive cell-specific expression of the human M2 muscarinic acetylcholine gene. J. Neurochem. 91, 88-98 (2004). IF₂₀₀₄ = 4.821.
• Belyaev ND, Wood IC, Bruce AW, Street M, Trinh JB, Buckley NJ. Distinct RE1 Silencing Transcription Factor (REST)-containing complexes interact with different target genes. J. Biol. Chem. 279, 556-561 (2004). IF₂₀₀₄ = 6.355.
• Wood IC, Belyaev ND, Bruce AW, Jones C, Mistry M, Roopra A, Buckley NJ. Interaction of the repressor element 1-silencing transcription factor, (REST), with target genes. J. Mol. Biol. 334, 863-874 (2003). IF₂₀₀₃ = 5.239.

Total publications (Web of Science 10.10.2024): 35
Hirsh index (Web of Science 10.10.2024): 19
Times cited (Web of Science 10.10.2024, excluding self-citations): 5775
Average impact factor (IF) per publication (based on IF in year of publication): 7.584

 Ph.D. (Doctoral) Theses (x3):

• Bora P. 'The role of p38-MAPKs in mouse preimplantation embryonic development: Regulating translation towards blastocyst maturation and lineage specification". (2021). University of South Bohemia, Faculty of Science, České Budějovice; Physiology & Developmental Biology/ Animal Physiology & Developmental Biology programme.
• Mihajlović AI. ‘The involvement of the Hippo signalling pathway in the first two cell-fate decisions of pre-implantation mouse embryo development’. (2017). University of South Bohemia, Faculty of Science, České Budějovice; Physiology & Developmental Biology/ Animal Physiology & Developmental Biology programme.
• Thamodaran V. ‘The role of p38-Mapk14/11 as an enabler of primitive endoderm (PrE) maturation and as a sensor of metabolism during mouse preimplantation embryo development’. (2016). University of South Bohemia, Faculty of Science, České Budějovice; Physiology & Developmental Biology/ Animal Physiology & Developmental Biology programme.

 Mgr. (Masters) Theses (x5):

• Hauserová A. 'Investigating functional p38-MAPK signalling during mouse blastocyst ICM cell-fate maturation'. (2023). University of South Bohemia, Faculty of Science, České Budějovice; Experimental Biology: Molecular & Cell Biology and Genetics programme.
• Kutsyna V. 'Assaying mitochondrial cell number, morphology and respiration status in the emerging three lineages of the preimplantation stage mouse embryo'. (2023). University of South Bohemia, Faculty of Science, České Budějovice; Experimental Biology: Molecular & Cell Biology and Genetics programme.
• Černá P. 'mTOR-regulated translation during the first wave of inner cell generation in mouse preimplantation embryos'. (2022). University of South Bohemia, Faculty of Science, České Budějovice; Physiology & Developmental Biology/ Animal Physiology & Developmental Biology programme.
• Pekl'anská M. 'Utilising CRISPR-Cas9 mediated genome editing to C-terminally tag endogenous gene loci with fluorescent protein fusions in preimplantation mouse embryos'. (2021). University of South Bohemia, Faculty of Science, České Budějovice; Physiology & Developmental Biology/ Animal Physiology & Developmental Biology programme.
• Kubíčková M. Investigation of the gene Dynactin subunit 2 (Dctn2) in regulating the frequency of asymmetric cell divisions during mouse preimplantation embryonic development. (2018). University of South Bohemia, Faculty of Science, České Budějovice; Physiology & Developmental Biology/ Animal Physiology & Developmental Biology programme.

Bc. (Bachelors/ B.Sc.) Theses (x9):

• Botnari F. Assaying the expression levels of Erk1 and Erk2 mitogen-activated-kinase, and Btbd18 gene mRNAs in various stages of preimplantation mouse embryo development (2024). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Bock N. Assaying the inter-cell heterogenous formation of intermediate filaments (specifically the KRT8 protein) in the development of the preimplantation mouse embryo (2024). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Hauserová A. Analyzing the role of p38 mitogen activated protein kinases and their effectors on mouse blastocyst maturation (2021). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Biology Programme.
• Gajovská T. Effects of mTOR and AMPK pathways on specification of inner cell mass (ICM) cell lineages during preimplantation development (2021). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Biology Programme.
• Moser F. Cloning useful recombinant cDNA/mRNA constructs to study cell cycle and Hippo-signalling in preimplantation mouse embryos (2020). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Pezelj S. Generating fluorescently tagged MARK2 fusion protein as a marker for the basolateral membranes of preimplantation stage mouse embryo blastomeres (2019). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Holzer MA. Assaying the effect of VEGFR2 receptor inhibition on preimplantation mouse embryo development (2018). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Schnabellehner SC. The role of Csf1 and Zfpm1 in the preimplantation mouse development (2016). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Jakesová M. Role of Csf1 during preimplantation mouse development (2014). University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Joint Biological Chemistry Programme.
• Ahiable JEA. Cloning candidate novel cell-fate genes (pre-implantation mouse development). (2012). University of South Bohemia, Faculty of Science, České Budějovice Czech Republic; Joint Biological Chemistry Programme.
• *Gstöttenmayer D. DNA separation by capilliary electrophoresis: a literature review (2011).   of South Bohemia, Faculty of Science, České Budějovice Czech Republic; Joint Biological Chemistry Programme.
  
  * literature review only

• Grant Agency of the Czech Republic (Grantová agentura České republiky, GA ČR), Panel P303; Cellular, Developmental & Evolutionary Biology (2020 - 2024).
• Hungarian National Research, Development & Innovation Office (NKFI), Developmental Life Sciences Panel, Budapest, Hungary (2019 & 2020).
• Medical Research Council (MRC), 58 Victoria Embankment, London, EC4Y 0DS, United Kingdom (x1; 2021, x2; 2022).
• Grant Agency of Charles University (Grantová agentura univerzita Karlova, GA UK), Prague, Czech Republic (x2; 2016, x2; 2018; x1 2023).
• Biotechnology and Biological Sciences Research Council (BBSRC), Polaris House, North Star Avenue, Swindon SN2 1UH, United Kingdom (x1; 2016, x1; 2018).

• Review Editor at Frontiers in Cell and Developmental Biology (2021 onwards). Based at Frontiers Media SA, Avenue du Tribunal Federal 34, 1005 Lausanne, Switzerland. 
• Member of the Editorial Board of Reproductive BioMedicine Online (2013 onwards). Based at Duck End Farm, Park Lane, Dry Drayton, Cambridge, CB23 8DB, United Kingdom. Published on behalf of Reproductive Healthcare Ltd. by Elsevier Ltd.
• Ad hoc reviewer for scientific journals; Development, Reproduction, Reproductive BioMedicine Online, PLoS Biology, Nucleic Acids Research, iScience, Scientific Reports, International Journal of Molecular Sciences, Biochemical Journal and Bioscience Horizons.

• Habilitation thesis (Associate Professor). Anonymous, Canadian Research Chairs (2020) – strictly confidential details available on request.
• Habilitation thesis (Associate Professor/docent). MVDr. Martin Anger, C.Sc., Department of Histology & Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic (2019).

Examination appointments at Faculty of Science, University of South Bohemia in České Budějovice:

• summer 2010 - present: appointed to the examining committees for bachelors and masters level degree defences.
• summer 2010 - present: appointed to the examining committees for doctorate entrance and final state exams - including as a member of the Specialist Board (Developmental Biology) within the Integrative Biology  study programme, since 2021. 
  
  

 Specific & external Ph.D. (Doctoral) theses examinations:

• Horvat F. 'Bioinformatic analysis of RNA dynamics during mammalian oocyte-to-embryo transition'. (2023). Charles University in Prague, Faculty of Science, Prague, Czech Republic.
• Lilao-Garzon J. 'Effect of maternal age and metabolic syndrome on preimplantation embryo development'. (2023). University of Las Palmas in Gran Canaria, University Institute for Biomedical and Health Research, Las Palmas, Spain.
• Lopez-Contreras AJ. ‘Characterization of the ODCp gene and the novel related gene, inhibitor of antizymes 2/AZIN2’. (2008). University of Murcia, Faculty of Medicine, Department of Biochemistry, Murcia, Spain.

Specific & external M.Sc. (Masters) theses examinations:

• Gupta M. ‘Finding direct targets of the transcription factor Prospero in the central nervous system (CNS) of the Drosophila melanogaster. (2005). University of Cambridge, Department of Biochemistry, Cambridge, United Kingdom.

• From 2022: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Programme guarantor and head for the newly awarded grant ‘National Renewal Plan for Higher Education 2022-2024; creation of new study programmes in progressive fields’; as awarded by the Czech Ministry of Education, Youth and Sports’ (25m CZK). To redevelop a new Master’s study programme to reflect appropriate training within the ‘Faculty of Science’ and ‘Department of Molecular Biology and Genetics’ to meet modern employment requirements and opportunities; with specific reference to large data set generation and analyses using modern ‘-omics’ techniques (subject to study programme content accreditation by the Ministry – due start date: September 2024).

• From 2021: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Teaching and mentoring a practical  laboratory based teaching class for the 'Advanced Methods of Cell & Molecular Biology (KMB603 & KMB603E)' practical courses, taught in the summer semesters, for MSc. and Ph.D. students. Class entails three days of mouse ovary and, oviduct and uterus dissection, preimplantation embryo recovery, micro-manipulation by microinjection of RNAi and recombinant mRNAs, in vitro culture, fixation and staining and confocal microscopy & image analysis. Course guarantor Mgr. Lenka Gahurová Ph.D.

• From 2018: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Teaching and mentoring a practical  laboratory based teaching class for the 'Methods of Cell Study (KME614 )' and 'Developmental Biology of Animals and Humans (KMB617)' study courses/programmes, taught in the winter and summer semesters, respectively. Class entitles three hours of mouse ovary and, oviduct and uterus dissection, preimplantation embryo recovery and in vitro culture; with theory on early mouse development, cell lineage formation and general reproduction. Course guarantor Mgr. Hana Sehadová Ph.D.

• 2014-2023: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Establishment and lecturing the English taught undergraduate course (operated as part of the ‘Joint Biological Chemistry’ programme with Johannes Kepler University, Linz, Austria) entitled ‘Introduction to Genomics (KMB358)’. Three times two hour lectures (2014 – present, not including ad hoc revision classes not listed in STAG database), taught in the summer semester. Also acting as course guarantor and attracting lecturers from both within and outside the university and faculty (e.g. internally, Mgr. Aleš Horák Ph.D., Mgr. Lenka Gahurová Ph.D. and Mgr. Filip Husník; externally, Mgr. Jan Pačes Ph.D. & RNDr. Miluše Hroudová Ph.D. – Institute of Molecular Genetics/ IMG, Czech Academy of Science, Prague, Czech Republic).

• From 2011: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Lecturing the English taught undergraduate course (operated as part of the ‘Joint Biological Chemistry’ programme with Johannes Kepler University, Linz, Austria) entitled ‘Molecular Biology and Genetics I (KMB758)’. Ten times two hour lectures (2010 – 2014) and eight times two hour lectures (2015 – present), not including ad hoc revision classes not listed in STAG database, taught in the winter semester. Also acting as course guarantor, recruiting other faculty members to teaching roles (e.g. Mgr. Lenka Gahurová Ph.D.).

• 2011-2018: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic (in cooperation with Johannes Kepler University, Linz, Austria); Acted as course guarantor responsible for overall running/planning of the cross-boarder (CZ-A)  Bioinformatics Programme.

• 2011 & 2015: University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic; Ad hoc lecturing on the English taught Masters research degree course entitled ‘Advanced methods in Molecular Biology (KMB603)’. One two hour lecture called ‘Introduction to Microarrays’, taught in the summer semester. Course guarantor, Mgr. Doležel David Ph.D.

• 2008-2009: University of Cambridge, Cambridge, United Kingdom; Lecturing ‘part II’ undergraduate students in preimplantation embryo development and Assisted Reproductive Technology/ ART module entitled ‘The mouse as a model system’. 2.5 hours. Course guarantor, Prof. Martin H. Johnson (This email address is being protected from spambots. You need JavaScript enabled to view it.).

Polish Society of Reproduction, Warszaw, Poland. ´Post translational regulation of cell fate acquisition during preimplantation mouse embryo development: the emerging role of mTOR and p38-MAPK signalling.´ (September 2024) - Invited speaker.

Charles University, Faculty of Sciences (incorporating Masters student programme invite speaker seminar series); Prague, Czech Republic. ‘How to build a blastocyst: regulation of cell fate acquisition during mouse preimplantation embryo development.’ (April 2024) – invited speaker.

Visegrád Group Society for Developmental Biology (V4SDB) Third Meeting; Warsaw, Poland. 'Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and 7mG-cap dependent translation at the 8- to 16-cell transition.' (September 2023) - selected abstract oral presentation.

Institute of Animal Physiology and Genetics (IAPG); Liběchov, Czech Republic (IAPG 60^th anniversary symposium) ‘How to build a blastocyst; regulation of cell fate acquisition during mouse preimplantation embryo development’. (May 2023) – invited speaker.

University of Las Palmas in Gran Canaria (Spain); ‘Insights into the regulation of cell fate acquisition during mouse preimplantation embryo development’. (May 2023) – invited speaker.

University of Ostrava, Faculty of Sciences; Ostrava, Czech Republic ‘How to build a blastocyst; regulation of cell fate acquisition during mouse preimplantation embryo development’. (December 2022) – invited speaker.

University of South Bohemia (Scientific Board); České Budějovice, Czech Republic. ‘doc. Alexander W. Bruce, Ph.D.: A career summary’. (March 2022) – PROFESSORSHIP APPOINTMENT PROCEDURE (oral presentation).

Charles University, Faculty of Sciences (invited speaker seminar series, incorporating Masters student programme); Prague, Czech Republic. ‘Preimplantation mouse embryo development; the mechanics of building the blastocyst.’ (March 2022) – invited speaker.

University of South Bohemia, Faculty of Science (Scientific Board); České Budějovice, Czech Republic. ‘How to build a blastocyst: regulation of cell fate acquisition during mouse preimplantation embryo development’. (November 2021) – PROFESSORSHIP NOMINATION LECTURE (oral presentation).

Visegrád Group Society for Developmental Biology (V4SDB) Second Meeting; Szeged, Hungary. 'p38-MAPK-mediated translation regulation primes blastocyst development and is required for primitive endoderm differentiation in mice.' (September 2021) - selected abstract oral presentation and poster.

Czech and Slovak Stem Cell Biology Meeting; Prušánky, Czech Republic. 'The role of p38-MAPKs in specifying the primitive endoderm lineage of mouse blastocyst embryos.' (October 2020 - postponed due to Coronavirus pandemic) - invited speaker.

Hungarian Molecular Life Science Conference; Eger, Hungary. 'Deciphering the nodal role of p38 family of mitogen-activated protein kinases (MAPKs) towards specifying primitive endoderm (PrE) fate in the inner cell mass (ICM) of late-blastocyst stage mouse preimplantation embryo'. (March 2019) - selected abstract oral presentation.

Institute of Genetics & Animal Breeding (Polish Academy of Sciences): Jastrzbeic (near Warsaw), Poland. 'A question of fate: cell lineage derivation during preimplantation mouse embryogenesis'. (May 2018) - invited talk (oral presentation).

University of South Bohemia, Faculty of Science; České Budějovice, Czech Republic. ‘Regulation of mammalian cell-fate: Insights from genomics-based investigation of transcriptional regulation and chromatin structure & studies of preimplantation mouse embryogenesis’. (February 2018) – HABILITATION DEFENCE LECTURE (oral presentation).

Masaryk University, Faculty of Science; Brno Czech Republic. ‘A question of fate; cell lineage derivation during preimplantation mouse embryogenesis’. (January 2018) – invited talk (oral presentation).

University of South Bohemia, Faculty of Science; České Budějovice, Czech Republic. ‘Needing to be different; A summary of cell-fate acquisition in preimplantation mouse embryos.’ (October 2017) - PEDAGOGICAL HABILITATION LECTURE (oral presentation).

British Society for Developmental Biology (BSDB) Autumn Meeting; Edinburgh, United Kingdom. ‘p38 mitogen activated kinases (Mapk14/11) occupy a regulatory node governing entry into mouse embryo primitive endoderm differentiation’. (August 2016) – selected abstract oral presentation and poster (O04 & P21).

University of Manchester, Division of Developmental Biology & Medicine; Manchester, United Kingdom. ‘A question of fate! Cell lineage derivation during preimplantation mouse embryogenesis’. (November 2015) – invited talk (oral presentation).

Czech RNA Club Annual Meeting; University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic. ‘The first two cell-fate decision of preimplantation mouse embryo development are not functionally independent’. (September 2015) – invited talk (oral presentation).

Vita Nova International Education Event (Assisted Reproductive Technologies/ ART); Bratislava, Slovakia. ‘Generating different genetic expression patterns in the early embryo; insights from the mouse model’. (September 2013) – invited talk (oral presentation).

Futures in Reproduction Meeting; Celebrating the award of the 2010 Nobel Prize for Physiology or Medicine to Professor Sir Robert Edwards; Churchill College, University of Cambridge, Cambridge, United Kingdom. ‘Generating different genetic expression patterns in the early embryo; insights from the mouse model’. (December 2012). – invited talk (oral presentation).

Institute of Molecular Genetics (IMG); Czech Academy of Sciences, Prague, Czech Republic. ‘Understanding cell-fate decisions in pre-implantation mouse embryogenesis’. (November 2012). – invited talk (oral presentation).

Charles University, Third Medical Faculty; Prague, Czech Republic. ‘Exploring the role of the Cdx2 gene during the first cell-fate choices of mouse embryo development’. (March 2012). – invited talk (oral presentation).

Institute of Molecular Genetics (IMG); Czech Academy of Sciences, Prague, Czech Republic. ‘Understanding cell-fate decisions in pre-implantation mouse embryogenesis’. (November 2011). – invited talk (oral presentation).

Institute of Animal Physiology and Genetics (IAPG); Czech Academy of Sciences, Liběchov, Czech Republic. ‘A complex state of affairs: Cdx2 and preimplantation mouse development’. (December 2010). – invited talk (oral presentation).

University of Warsaw, Faculty of Biology, Department of Embryology; Warsaw, Poland. ‘A complex state of affairs: Cdx2 and preimplantation mouse development’. (October 2010). – invited talk (oral presentation).

University of South Bohemia, Faculty of Science, Department of Molecular Biology; České Budějovice, Czech Republic. ‘Giving Functional Genomics a REST’. (May 2009). – invited talk (oral presentation).

The Wellcome Trust Sanger Institute, Wellcome Trust Advanced Courses Workshop (microarrays & the transcriptome); Welcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom. ‘The Application of Functional Genomics: REST, a case study’. (April 2009). – invited talk (oral presentation).

National Centre for Oncological Investigations (CNIO), Madrid, Spain. ‘Giving functional genomics a REST’. (February 2009). – invited talk (oral presentation).

University of Murcia, Faculty of Medicine; Murcia, Spain. ‘Functional characterisation and target gene identification of the transcriptional repressor REST.’ (February 2007). - invited talk (oral presentation).

Anglo-Czech Partnerships in Science Cancer Symposium (sponsored by the British Council); Prague, Czech Republic ‘Using genomic DNA microarrays to identify regulatory elements in the human Stem Cell Leukaemia (SCL) locus’. (March 2005) - selected abstract (oral presentation).

London Chromatin Club Meeting (sponsored by Abcam); London, United Kingdom. ‘Genomic DNA Microarrays: Applications in Chromatin Research (ChIP:Chip)’. (January 2005). – invited speaker/ oral presentation.

Inaugural Chromatin Structure and Function Meeting (sponsored by Abcam); Cancún, Mexico. ‘Identification of transcription factor binding sites and chromatin structural domains in the human genome using DNA genomic microarrays’. (November 2004). – selected abstract (oral presentation), plus poster (P6).

British Biochemical Society Meeting; BioScience; Glasgow, United Kingdom. ‘The transcriptional repressor REST coordinates the regulated secretory pathway’. (July 2004). – selected abstract (oral presentation), plus poster A110).

International Society for Developmental Biology (ISDB) Meeting; Algarve, Portugal (October 2022) – x2 LEMDB posters.
Visegrád Group Society for Developmental Biology (V4SDB) Student Practical Summer School (Models of Early Embryological Research);  Balaton Limnological Research Institute, Tihany, Lake Balaton, Hungary. (June 2022) - course faculty member & lecturer.
Visegrád Society for Developmental Biology (V4SDB) Second Meeting; Biology Research Centre, Szeged, Hungary (September 2021) – x4 LEMDB lab posters.
European Developmental Biology Congress (EDBC); Alicante, Spain. (October 2019) - x4 LEMDB posters.
Visegrád Society for Developmental Biology (V4SDB) Inaugural Meeting; Masaryk University, Brno, Czech Republic (September 2018) – x3 LEMDB lab posters.
British Society for Developmental Biology (BSDB) Spring Meeting; Warwick University, Warwick, United Kingdom. (April 2018).
British Society for Developmental Biology (BSDB) Joint Autumn Meeting (with the Portuguese and Spanish Developmental Biology Societies); Alfamar Resort, Algarve, Portugal. (October 2015) – x2 LEMDB lab posters.
The 6^th EMBO Meeting – Advancing Life Sciences; Birmingham, United Kingdom. (September 2015) – poster presentation (EMBO confirmed).
Inside-Outside, Challenges and Prejudices; Celebrating the work of Prof. Martin H. Johnson; University of Cambridge, Cambridge, United Kingdom. (September 2015).
Czech Cytoskeleton Club XXIII Annual Meeting; Vranovská Ves, Czech Republic. (May 2015).
Czech Cytoskeleton Club XXII Annual Meeting; Vranovská Ves, Czech Republic. (May 2014).
EMBO Workshop. Oocyte maturation and fertilization: Lessons from canonical and emerging models; Banyuls-sur-Mer, France. (June 2013).
Gurdon Institute 21^st Anniversary Symposium; Cambridge, United Kingdom. (June 2013).

• Visegrad Group Society for Developmental Biology (V4SDB) Third Meeting; Warsaw, Poland. (September 2023). Member of general organising committee. https://v4sdb2023.pl/welcome_message
  International Society of Developmental Biologists, 19th International Congress of Developmental Biology; Salagados Palace, Algarve, Portugal (October 2022). International Scientific Committee Member (re: scientific programme) https://www.isdb2021.com/  (committee membership details - https://www.isdb2021.com/committees)
• Visegrád Group Society for Developmental Biology (V4SDB) Student Practical Summer School (Models of Early Embryological Research);  Balaton Limnological Research Institute, Tihany, Lake Balaton, Hungary. (June 2022). Course faculty member & lecturer (>35 research students students – preimplantation mouse embryo, with dr. hab. Anna Piliszek - Institute of Genetics & Animal Breeding, Polish Academy of Sciences, dr. hab. Anna Ajduk – Faculty of Biology, University of Warsaw, Poland and MVDr. Dušan Fabian, Slovak Academy of Sciences). http://v4sdb.org/2022-v4sdb-summer_school 
• Visegrád Group Society for Developmental Biology (V4SDB) Second Meeting - incorporating Day 0 student practical workshop; Szeged, Hungary. (September 2021). Junior member of main conference organising committee & co-lecturer/ demonstrator and co-main organiser (with dr. hab. Anna Piliszek - Institute of genetics & Animal Breeding, Polish Academy of Sciences) of 'Preimplantation mouse embryo recovery and in vitro culture/ manipulation practical class' (>30 research students). http://v4sdb.org/second-v4sdb-meeting-2-1 and https://www.v4sdbszeged.com/ 
• Visegrád Group Society for Developmental Biology (V4SDB) Inaugural Meeting; Brno, Czech Republic. (September 2018). Member of Czech organising committee. https://webcentrum.muni.cz/visegrad2018 
• University of South Bohemia Confocal Microscopy and Image Analysis Workshop (2012 & 2015). University of South Bohemia, Faculty of Science, České Budějovice. Head of the organisation committee (recruiting Mr. Alex Sossick, Head of the Imaging Facility at the Gurdon Institute, University of Cambridge, United Kingdom and course instructors from the companies BitPlane/Imaris image analysis and Olympus microscopes) for one week intensive practical workshop and trouble-shooting clinic, aimed at faculty Ph.D. students.
• Annual Wellcome Trust Advanced Course; Microarrays and the Transcriptome (2005-2007). Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, United Kingdom. Teaching assistant. Course administrators; David Vetrie Ph.D. (This email address is being protected from spambots. You need JavaScript enabled to view it.) and Mrs. Yvonne Hitching (This email address is being protected from spambots. You need JavaScript enabled to view it.).

• Annual Department of Molecular Biology (& Genetics – since 2016) Scientific Image Competition (2011 onwards); Establishment and organisation of an annual competition within the Department of Molecular Biology (& Genetics), Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic, to showcase exquisite scientific images, judged and awarded on the basis of both aesthetics and scientific merit, generated within the department within the last calendar year. Generated images used for promotional materials. See archived entrant galleries.
• Founder member of the organising committee for the establishment of a Visegrád Group (Poland, Czech Republic, Hungary and Slovakia) Society for Developmental Biology (V4SDB) - re-elected for 2024-2027 term as V4SDB Treasurer & Membership Secretary; Work begun in December 2016 with Polish (Anna Piliszek Ph.D., Institute of Genetics and Animal Breeding, Warsaw, Poland) and Czech colleagues (doc. RNDr. Marcela BuchtováPh.D., Masaryk University, Faculty of Science, Brno, Czech Republic) to establish of an international developmental biology society to sustainably support and promote the field in the Visegrád Group. For more information see the official webpage,  V4SDB facebook page and Twitter feed (@V4Sdb)

In accordance with the Scholarship Regulations of USB, the support for a given calendar year is paid in the form of a scholarship for outstanding research, development and innovation, or other creative results contributing to the deepening of knowledge to the PhD candidates, who meet the following conditions:

• The PhD candidate is in full-time study of the 2nd year or higher and is supervised by a key employee of the faculty*

• The PhD candidate has fulfilled all the obligations set by the ISP during the previous year and the annual report has been evaluated by the Specialist Board with a grade of "A"

• The study did not exceed the standard study period, i.e. 4 years

The amount of the scholarship for each calendar year is decided by the Dean. For the year 2024 it is set at CZK 3000 per month and the scholarship can be paid retroactively.

In České Budějovice, 20th February 2024

doc. RNDr. Eva Nováková, PhD.
Vice-Dean for Doctoral Studies

*key employee status is determined by the Dean of FSc USB