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doc. Mgr. Tomáš Doležal, Ph.D.

head of the Laboratory of Molecular Integrative Physiology in Drosophila

Faculty of Science, University of South Bohemia
Branišovská 31, 37005 České Budějovice
building B, ground floor, door number 193
 
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tel. +420 387772229
ORCID ID: 0000-0001-5217-4465


doc. Mgr. Tomáš Doležal, Ph.D.

Current possitions

since 2006
Group leader
since 2023
Member of the Scientific Board

Faculty of Science, University of South Bohemia, České Budějovice

since 2021
Chair of the Programme Board
since 2019
Guarantor of the doctoral study programme
since 2017
Guarantor of the master study programme

Previous possitions

2016-2021
Head of the Department of Molecular Biology and Genetics

Faculty of Science, University of South Bohemia, České Budějovice

2012-2014
Visiting assistant professor

Stanford University School of Medicine, USA

2003-2005
Laboratory of Prof. Peter Bryant

 University of California, Irvine, USA

2000-2003
Laboratory of Dr. Michal Žurovec

Institute of Entomology ASCR, České Budějovice

1999-2000
Laboratory of Prof. Peter Bryant

University of California, Irvine, USA

1996-1999
Laboratory of Molecular Genetics and DNA Diagnosis

1st Medical Faculty, Charles University, Prague

Education

2015
Habilitation (doc.) Molecular and Cell Biology and Genetics

Faculty of Science, University of South Bohemia, České Budějovice

2003
Ph.D., Molecular and Developmental Biology and Genetics

Faculty of Biology, University of South Bohemia, České Budějovice

1999
MSc., Genetics, Molecular Biology and Immunology

Faculty of Science, Charles University, Prague

Awards

2022
Rheos Short Talk Award

2nd Metabolism in Health and Disease Conference 2022, Cancun, Mexico

2017
Prize of the Learned Society of the Czech Republic

in the category of Researcher of the Year 2017 for the validation of the new concept of selfish immunity

2004
cena Barbary Burgess

for excellence in postdoctoral research at University of California, Irvine, USA. Project: Role of ADGF-A gene in Drosophila

International scientific activity

2012-2014
visiting assistant professor

Stanford University School of Medicine, USA (1,5 roku)

2003-2005
postdoctoral fellowship, Prof. Peter Bryant's lab

University of California, Irvine, USA

Research projects (principal investigator)

2020-2023
GAČR 20-09103S

Molekulární mechanismy privilegovaného přístupu aktivovaných imunitních buněk k energii u drozofily

2017-2019
GAČR 17-16406S

Vznik adenozinu jako signálu sobecké imunity

2012 – 2015
Marie Curie International Outgoing Fellowship 298186

Extracellular adenosine role in energetic metabolism during immune response

2012 – 2014
GAČR P305/12/0115

Model drozofily pro studium účinků extracelulárního adenozinu na mechanismy rezistence a tolerance k patogenům

2009 – 2011
GAČR 204/09/1463

Extracelulární adenozin jako stresový hormon v drozofile

2007 – 2010
GAČR 301/07/0814

Zkoumání úlohy kasein kinázy Iepsilon pro vznik rakoviny prsu

2006 – 2008
GAAV KJB501410602

Úlohy extracelulárního adenozinu při krvetvorbě a vrozené imunitě za použití drozofily jako modelu pro studium těchto úloh in vivo 

Scientific projects (hosting lab, mentor)

2023-2024
EMBO Postdoctoral Fellowship

Dr. Ellen McMullen
Immune system vs. Brain: Do metabolic privileges change upon infection?
(ALTF 23-2022)

2021-2023
MŠMT OPVVV, 02-20-079 MSCA IF IV

Dr. Michaela Fencková
The emergence of adenosine as a signal of selfish immunity
(ID CZ.02.2.69/0.0/0.0/20_079/0017633)

2020-2023
Marie Skłodowska-Curie grant

Dr. Michalina Kazek
Trehalose as a source for privileged immunity in Drosophila 
(H2020-WF-01-2018, ID 867430, IMMUNETREH)

Development projects (principal investigator)

2007
FRVŠ

Establishment of a joint laboratory for practical teaching of organic chemistry and molecular biology

2010-2013
Operational Programme Education for Competitiveness (OP VK)

Molecularization of biological disciplines at the Faculty of Science, University of South Bohemia, České Budějovice

Invited lectures

10/11/2017
Why is our immune system selfish?

Kužela seminar, Comenius University in Bratislava

22/9/2017
Molecular mechanisms connecting activation of immune cells with metabolic changes at systemic level

Immunity and Hematopoeisis Workshop, European Drosophila Research Conference 2017, London

23/2/2017
Energy privilege of activated immune system

Lecture for the Czech Immunological Society, Prague

22/11/2016
What did we learn from fruit flies about a selfish behavior of immune system?

Lecture for the Learned Society of the Czech Republic, Prague

21/5/2016
Adenosine as a signal of selfsh immune system - fruit fly model

ESID Eastern Spring School in Primary Immunodeficiencies, Třeboň

25/9/2022
Metabolism of privileged immune cells
Drosophila Blood Cell Biology Symposium, Strasbourg, France

Oral presentations at international conferences

5/2022
The origin of adenosine as a systemic metabolic regulator in privileged immune cells

2nd Metabolism in Health and Disease Conference, Cancun, Mexico

7/2019
Molecular regulations of metabolism during immune response in Drosophila. 

T. Dolezal, A. Bajgar, G. Krejcova, P. Nedbalova - Eighth International Symposium on Molecular Insect Science (Elsevier) 7-10 July 2019  Sitges, Spain

3/2014
Extracellular adenosine regulates complex host-pathogen interactions through the energy release for the immune response

T. Dolezal, A. Bajgar, K. Kucerova, L. Jonatova, DS Schneider - 55th Annual Drosophila Research Conference, 26.–30.3. 2014, San Diego, USA

11/2009
The discs overgrown (dco) mutant - a fly model for the role of Casein Kinase Iε in breast cancer

T. Dolezal, J. Matulova, K. Kucerova, E. Kubala, PJ Bryant - 21st European Drosophila Research Conference, Nice, France

7/2003
Adenosine Deaminase-related Growth Factors (ADGFs) and extracellular adenosine mediate the juvenile hormone response in Drosophila

Great Barrier Reef Drosophila Conference, Cairns, Australia

Membership in committees

since 2023
Member of the Scientific Board

Faculty of Science, University of South Bohemia, České Budějovice

2007-2009
Member of the Board

Grant agency of University of South Bohemia

since 2007
Member of the regional committee

Secondary school vocational activities (SOČ)

Publications

  • Doležal T. (2023) How to eliminate pathogen without killing oneself? Immunometabolism of encapsulation and melanization in Drosophila. Frontiers in Immunology. Dec 6:14:1330312. doi: 10.3389/fimmu.2023.1330312. eCollection 2023.
  • Krejčová G., Morgantini C., Zemanová H., Lauschke V.M., Kovářová J., Kubásek J., Nedbalová P., Kamps-Hughes N., Moos M., Aouadi M., Doležal T. & Bajgar A. (2023) Macrophage-derived insulin antagonist ImpL2 induces lipoprotein mobilization upon bacterial infection. The EMBO Journal. Dec 1;42(23):e114086. doi: 10.15252/embj.2023114086. Epub 2023 Oct 9.
  • Bajgar A,  Krejčová G, Doležal T (2021) Polarization of Macrophages in Insects: Opening Gates for Immuno-Metabolic Research. Front Cell Dev Biol, 15 February 2021 | https://doi.org/10.3389/fcell.2021.629238 (IF=5.2)
  • Doležal TNedbalová P, Krejčová G, Kazek M, Lehr K, Chodáková L, Strych LDolejšková T, Bajgar A (2020) Privileged immune cell upon activation – how it changes its own metabolism and metabolism of the whole organism.  figshare POSTER DOI: 10.6084/m9.figshare.12144831.v1
  • Krejcova G, Danielova A, Nedbalova P, Kazek M, Strych L, Chawla G, Tennessen JM, Lieskovská J, Jindra M, Dolezal T, Bajgar A (2019) Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense. eLife 14;8. pii: e50414. doi: 10.7554/eLife.50414. (IF=7.5)
  • Bajgar A, Salon I, Krejcová G, Dolezal T, Jindra M, Stepanek F (2019) Yeast glucan particles enable intracellular protein delivery in Drosophila without compromising the immune system. Biomater Sci 30. doi: 10.1039/c9bm00539k. (IF=5.2)
  • Dolezal T, Krejcova G, Bajgar A, Nedbalova P, Strasser P (2019) Molecular regulations of metabolism during immune response in insects. Insect Biochem Mol Biol 109:31-42. doi: 10.1016/j.ibmb.2019.04.005. (IF=3.9)
  • Bajgar A, Dolezal T (2018) Extracellular adenosine modulates host-pathogen interactions through regulation of systemic metabolism during immune response in Drosophila. PLoS Pathog 14(4): e1007022 (IF=6.6)
  • Dolezal T (2015) - Adenosine: a selfish-immunity signal? Oncotarget - Immunology and Microbiology Section 6 (32), 32307-32308 (IF=6.3)
  • Bajgar A, Kucerova K, Jonatova L, Tomcala A, Schneedorferova I, Okrouhlik J, Dolezal T (2015) Extracellular Adenosine Mediates a Systemic Metabolic Switch during Immune Response. PLoS Biol 13(4): e1002135 (IF=11.8)
  • Novakova M and Dolezal T (2011). Expression of Drosophila adenosine deaminase in immune cells during inflammatory response. PLoS ONE 6(3): e17741 (IF=3.2)
  • Fenckova M, Hobizalova R, Fric Z, Dolezal T (2011). Functional characterization of ecto-5’-nucleotidases and apyrases in Drosophila melanogaster. Insect Biochem Mol Biol 41(12): 956-967 (IF=3.4)
  • Zuberova M, Fenckova M, Simek P, Janeckova L, Dolezal T (2010). Increased extracellular adenosine in adenosine deaminase deficient flies activates a release of energy stores leading to wasting and death. Dis Model Mech 3(11-12): 773-84 (IF=4.9)
  • Dolezal T, Kucerova K, Neuhold J, Bryant PJ (2010). Casein kinase I epsilon somatic mutations found in breast cancer cause overgrowth in Drosophila. Int J Dev Biol 54: 1419 – 1424 (IF=2.8)
  • Foldynova-Trantirkova S, Sekyrova P, Tmejova K, Brumovska E, Bernatik O, Blankenfeldt W, Krejci P, Kozubik A, Dolezal T, Trantirek L, Bryja V. (2010). Breast cancer specific mutations in CK1epsilon inhibit Wnt/beta-catenin and activate Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration. Breast Cancer Res 12(3): R30 (IF=5.5)
  • Dolezal T, Dolezelova E, Zurovec M, Bryant PJ (2005). A Role for Adenosine Deaminase in Drosophila Larval Development. PLoS Biol 3(7): e201 (IF=11.8)
  • Dolezelova E, Zurovec M, Dolezal T, Simek P, Bryant, PJ (2005). The emerging role of adenosine deaminases in insects. Insect Biochem Mol Biol 35(5): 381-9 (IF=2.8)
  • Dolezal T, Gazi M, Zurovec M, Bryant PJ (2003). Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila. Genetics 165: 653-666 (IF=4.3)
  • Zurovec M, Dolezal T, Gazi M, Pavlova E, Bryant PJ (2002). Adenosine deaminase-related growth factors stimulate cell proliferation in Drosophila by depleting extracellular adenosine. PNAS 99(7): 4403-4408 (IF=10.9)

Teaching activities at the University of South Bohemia

Teaching of courses:
Fundamentals of Cell Biology (KMB/023) – guarantor and lecturer

Cellular and Molecular Biology and Genetics I (KMB/758) - guarantor and lecturer

Advanced methods in Molecular Biology (KMB/603) - guarantor and lecturer

2006-2016 guarantor and lecturer (100%) of “Foundation of modern biology” (undergraduate course)
2007-2010 guarantor and lecturer (100%) of “Molecular biology and Genetics” (undergraduate course in English for international program Biological Chemistry)
2008-2009 guarantor and lecturer (20%) of “Cell regulation and signaling” (graduate course in English)
2010-2012 guarantor and lecturer (100%) of “Basic methods in molecular biology” (undergraduate course)
since 2014 lecturer (50%) of “Developmental biology” (undergraduate course)
since 2017 guarantor and lecturer (100%) of "Introduction to Cell Biology" - mandatory course for Czech undergraduate Biology programme and undergraduate course in English for international program Biological Chemistry
since 2017 guarantor of "Methods in Molecular Biology" (undergraduate course in English for international program Biological Chemistry)

Supervision of students and postdocs

2 Postdocs / 4 Ph.D. students / 4 Master Students / 19 Bachelor Students

Ph.D. theses:
Milena Novakova, 2011 - "Regulation of extracellular adenosine level by ADGF-A in Drosophila melanogaster"
Michaela Fenckova, 2012 - "Role of extracellular adenosine in Drosophila"
Monika Zuberova, 2012 - "The role of adenosine signaling pathway in regulation of metabolic reserves"

Master degree theses:
Tomas Gregor, 2015 - "Tissue specific mutagenesis with TAL nucleases using Drosophila melanogaster as a model" (in Czech)
Lucie Jonatova, 2013 - "The Role of Dco in Drosophila Haematopoiesis”
Jana Matulova (Neuhold), 2009 - "Study of the Role of Casein Kinase I epsilon in BreastCancer Using Drosophila as a Model"

Supervised Bachelor students:
David Opavsky, Jana Kadlecova, Tomas Krucek, Radka Hobizalova, Eugen Kubala, David Hartmann, Sarka Pospisilova, Tamara Aigner, Bianca Spitzbart, Marek Renner, Kristina Preur, Tomas Gregor, Jiri Stros, Paul Strasser, Klara Kopicova, Thomas Stehrer, Barbara Jetzinger, Lukas Strych, Katharina Lehr

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